Random Association among Alleles in Clonal Populations ofSclerotinia sclerotiorum

Abstract

Multiple loci identified in DNA fingerprints were used to test for random association in two agricultural populations ofS. sclerotiorum.In linkage disequilibrium tests among pairs of loci with frequencies between 0.1 and 0.9, 44.5 and 80.5% of pairs of loci were consistent with random association in the clone-corrected samples of the Canadian canola and the North Carolina cabbage populations, respectively. In estimates of corrected (Bonferroni)Pvalue, 70.66 and 98.89% of pairs of loci were in random association. All four possible genotypes for each pair of loci were observed in the Canadian canola sample, consistent with random association among loci. In multilocus association tests across all loci, however, significant association was observed in both populations. In the Canadian canola population, 40 possible heterokaryons were identified. Our data suggest that populations ofS. sclerotiorumare predominantly clonal and that occasional genetic exchange and recombination, and not mutation alone, may be a source of new genotypes.