Abstract
Walnut oligopeptides (WOPs) intake is associated with the augment of the antioxidant defense system and immune system. The chief object of this study is to evaluate the radioprotective effect of walnut oligopeptides extracted from walnut seed protein against 60Coγ-irradiation induced damage in mice. Female BALB/c mice were administered WOPs through drinking water for 14 days until a single dose of whole-body 60Coγ-irradiation. The 30-day survival test was carried out in the first group (8 Gy), and the other two groups (3.5 Gy) were sacrificed at 3 days and 14 days post-irradiation. Blood and organ samples of mice in the three groups were collected, the histopathological analysis and immunohistochemistry were conducted. The number of peripheral blood leukocytes, bone marrow DNA content, inflammatory cytokines, antioxidant capacity, and intestinal permeability were measured. We found that the administration of WOPs augmented antioxidant defense system, accelerated hematopoietic recovery and showed the significant trend toward higher survival rate and less weight loss compared with non-administrated control mice. In addition, WOPs administration appeared to be important to limit IR-induced splenocyte apoptosis and inflammatory cascade as well as reduce intestine epithelial barrier dysfunction and promote epithelial integrity. These results suggest that pre and post-treatment of WOPs may help to ameliorate acute damage, which is induced by ionizing radiation in mice and accelerate its recovery.
Highlights
Ionizing radiation (IR) produces a large amount of reactive oxygen species (ROS), which has harmful effects on cellular macromolecules like DNA, lipids, proteins, and inducing a series of biological consequences including cell apoptosis, inflammation, autoimmune reaction, causing impairment of several organs and systems and even death
Whereas the treatment with Walnut oligopeptides (WOPs) significantly increased the level of IL-6 and decreased the level of tumor necrosis factor-α (TNF-α) in serum at both 3 and 14 days postirradiation
To determine whether intestinal mucosa was one of the targets for WOPs to exert its protective role, we examined the histology on 3 days and 14 days postirradiation
Summary
Ionizing radiation (IR) produces a large amount of reactive oxygen species (ROS), which has harmful effects on cellular macromolecules like DNA, lipids, proteins, and inducing a series of biological consequences including cell apoptosis, inflammation, autoimmune reaction, causing impairment of several organs and systems and even death. Reducing stress-induced barrier changes may have a therapeutic benefit [3,4,5]. Reducing stress-induced barrier has a negative effect on antioxidant system, could induces the decreased level of glutathione (GSH). As the exposure to IR, especially medical system, could induces the decreased level of glutathione (GSH) and the activity of antioxidant exposure, is regularly seen in our daily life, it becomes of great importance to seek for safe and enzymes [6,7]. Our previous data suggested that ginseng oligopeptides exhibits effective effective therapeutic effects on attenuating irradiation-induced hematopoietic, gastrointestinal, and therapeutic effects on attenuating irradiation-induced hematopoietic, gastrointestinal, and oxidative oxidative injury in mouse model by reducing systematic inflammatory and metabolic disorders [5]. WOPs against the total body-radiation induced harmful effects in mice.
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