Abstract
Abstract. Antibodies against prostaglandins (PG)F2α, E1 and E2 were obtained in rabbits immunized with respectively PG F2α, PG E1 and PG E2 conjugated to bovine serum albumin by carbodiimide. A radioimmunoassay capable of measuring 7 pg of PG Fα, 2 pg of PG E2 and 14 pg of PG E1 in human peripheral plasma is described. Plasma samples (pH 3, citric acid) are extracted with cyclohexane: ethyl acetate, 1:1 and then chromatographed on silicic acid columns to separate the prostaglandins into three fractions: fraction I, PG A, PG B and some unknown immunoreactive compounds; fraction II, PG E and fraction III, PG Fα. The recovery is 80 % ± 6.2. Mean plasma levels in adults of PG Fα and PG E, expressed in pg/ml: ‐PG Fα 12 ± 2.8 (n = 25 men), 8 ± 2.3 (n = 18 women, follicular phase), 7 ± 1.4 (n= 18 women, luteal phase). ‐PG El 40.5 ± 7.6 (n = 13 men), 38 ± 17.1 (n = 10 women). ‐PG E2 4.5 ± 1 (n = 12 adult subjects).The major characteristics of the method described herein are the following: ‐ a large volume of plasma has to be processed (10 ml or more for PG Fa and PG E1, 5 ml or more for PG E2). ‐ a chromatographic step is necessary to separate the different prostaglandins which makes it possible to circumvent problems of immunological cross reactivity and interference with unknown immunoreactive compounds. ‐ great care has been taken in collection of blood samples, especially to insure complete removal of blood cells namely platelets.
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