Abstract
目的：探索X线诱导食管鳞癌细胞内HOX转录反义RNA（HOX transcript antisense RNA,HOTAIR）及上皮间质化相关因子Snail,E-钙黏蛋白（E-cadherin）表达的时间剂量效应,方法：0、2、4、6、8 Gy X射线分别照射食管鳞癌Eca109细胞后,分别于0、2、4、8、16、24 h提取细胞内总RNA及蛋白,实时定量PCR检测（real-time quantitative PCR,qRTPCR）及Western blot检测相应指标mRNA及蛋白的表达水平.结果：Eca109细胞接受X线照射后继续培养24 h后发现,随X线照射剂量渐增大（0→2→4→6→8 Gy）,HOTAIR与Snail表达水平逐渐升高：HOTAIR mRNA,Snail mRNA在6 Gy与8 Gy照射组表达水平显著高于0 Gy组,Snail蛋白在4 Gy照射组即有显著升高;而E-cadherin mRNA与其蛋白表达水平逐渐降低,6 Gy与8 Gy照射组显著低于0 Gy组,均P〈0.01.细胞接受8 Gy X线照射后,伴随照射后培养时间延长（0→2→4→8→16→24 h）细胞内HOTAIR,Snail表达水平逐渐升高：与0 Gy组相比,细胞培养至第8小时HOTAIR mRNA表达水平显著升高,培养至第16小时Snail mRNA与其蛋白表达水平显著升高;而E-cadherin mRNA与其蛋白伴随照射后培养时间延长表达水平逐渐降低,培养至第8小时E-cadherin与其蛋白表达水平即显著低于0 Gy组（均P〈0.01）.结论：X射线诱导HOTAIR,Snail及E-cadherin的异常表达呈时间和剂量依赖性,三者可能共同参与肿瘤细胞放疗抵抗形成.
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