Abstract
Rab27b is reported to associate with the development and progression of several types of human cancers. However, the relationship between Rab27b expression and the clinical characteristics of lung adenocarcinoma (LUAD) is rarely explored. In this present study, the TCGA database was consulted, followed by one-step quantitative reverse transcription polymerase chain reaction (qPCR), Western blot, and immunohistochemistry (IHC) analyses in LUAD cell lines and tissue samples. Rab27b expression levels were statistically higher in LUAD cell lines and tissue samples compared with a noncancerous cell line and tissue samples (p < 0.05). Rab27b expression was statistically correlated with lymph node metastasis (p = 0.016) and TNM stage (p = 0.019). Survival analysis and Kaplan-Meier curve revealed that Rab27b expression (p = 0.006) and TNM stage (p = 0.027) were independently associated with the unfavorable overall survival of patients with LUAD. These results indicate that high expression of Rab27b correlates with malignant attributes of LUAD and Rab27b may be identified as a potential indicator of metastasis and prognosis for LUAD.
Highlights
Lung cancer is currently the most common cause of cancerrelated death worldwide [1]
Rab27b Expression in lung adenocarcinoma (LUAD) Cell Lines and Tissue Samples. quantitative reverse transcription polymerase chain reaction (qPCR) and Western blot analyses were performed in LUAD cell lines and tissue samples to further investigate Rab27b expression
The results of both qPCR and Western blot analyses demonstrated that Rab27b expression was significantly higher in LUAD cell lines than in a normal human bronchial epithelial cell line (HBE) cell line (Figures 2(a) and 2(b))
Summary
Lung cancer is currently the most common cause of cancerrelated death worldwide [1]. In China, there are over 700,000 newly LC cases and almost 600,000 LC patients died annually [2]. Non-small-cell lung cancer (NSCLC) accounts for approximately 85% of all LC cases, and lung adenocarcinoma (LUAD) is the most common histologic subtype [3]. For LUAD therapy, the effectiveness of traditional therapeutic strategies, including surgery, radiation, and chemotherapy, is unsatisfactory for they failed to accomplish significant survival benefits [4]. Identification of novel biomarkers with clinicopathological and prognostic significance is of great importance for LUAD management. An increasing number of studies have detected the crucial molecular alterations of NSCLC and screened several promising biomarkers for LUAD [5, 6]. There continues a remarkable demand for the identification of potential molecular biomarkers, and alternative treatment strategies are offered for LUAD
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