Abstract
BackgroundPseudomonas aeruginosa have been delineated for its disreputable charisma in nosocomial infections leading to pulmonary pneumonia. The recent developments in multi-drug resistance accelerate the need of alternative antibacterial strategies. In this study we analysed the efficacy of quorum quenching mediated bacterial silencing in effective immunological clearance upon pulmonary infected rat models.ResultsQuorum sensing inhibitor was extracted from Solanum torvum which is a native plant in Kerala, India. The in vitro pilot studies upon Pseudomonas aeruginosa strain jb07 revealed the ability of Solanum torvum root extract (STRE) to successfully inhibit quorum sensing controlled virulent factors such as chromogenesis and biofilm formation; though the bacterial growth was unaffected. In vivo analysis upon Sprague Dawley rats shown the potential of STRE in aiding successful bacterial clearance where in cent percentage of survival was evident that compared with untreated group. Subjects treated with STRE possessed natural immunological response with inflammation, MNC infiltration and BALT (bronchus associated lymphoid tissue) hyperplasia unlike the Ciprofloxacin treated comparative group.ConclusionsThe observations of this study suggests that STRE is not having any bactericidal activity rather its silencing the infectant in a system which in turn disables the virulence of bacteria and remain attenuated; therefore immune system get adequate time for successful countermeasures.
Highlights
Pseudomonas aeruginosa have been delineated for its disreputable charisma in nosocomial infections leading to pulmonary pneumonia
Effect of Solanum torvum root extract (STRE) upon in vitro attenuation and growth Hostile action of STRE upon quorum sensing controlled virulent properties such as chromogenesis and biofilm formation was found to be prominent against Pseudomonas aeruginosa strain jb07
Death of two rats (33.33%) in untreated group was recorded on 8th day of bacterial inoculation, none of the untreated subject survived till the end of study period as remaining subjects found dead on 12th day of challenge
Summary
Pseudomonas aeruginosa have been delineated for its disreputable charisma in nosocomial infections leading to pulmonary pneumonia. Bacteria interact with each other using chemical signalling molecules called autoinducers by a density dependent mechanism termed as quorum sensing. This enables the synchronization of large bacterial group activities such as pigment production, biofilm formation, swarming motility, toxin production and exozyme secretion [1]. In gram negative bacteria a vast diversity of LuxI/LuxR homologues genes are studied for its quorum sensing regulation, such as LuxI/LuxR in Vibrio harveyi regulating the bioluminance [5]; SmaI/ SmaR controlled production of carbapenem, prodigiosin, pectate lyase and cellulase in Serratia marcescens [6]; LasI/ LasR and RhiI/RhiR mediated virulence factors synthesis by Pseudomonas aeruginosa [7]. The studies on quorum sensing architecture suggest that it is possible to inhibit quorum sensing mediated bacterial virulence by suppressing bacterial communication
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
