Abstract
The QuickTox Kit for QuickScan Aflatoxin uses lateral flow technology and a reader-based system for quantitative determination of total aflatoxins. The performance of this assay was examined using corn samples naturally contaminated with aflatoxins in internal and independent laboratory evaluations and was judged against previously established acceptance criteria. Performance was evaluated for linearity, selectivity, matrix, robustness, and stability experiments. All data points in these studies fell within the ranges defined in the acceptance criteria. The assay exhibits linear dose response over the range tested, 0-100 ppb, with R2 values exceeding 0.98. RSDr values for results ranged from 4.7 to 17.7% across all tested levels. The four major aflatoxin types in corn are detected in the assay, with highest sensitivity for the most prevalent type, B1. Assay results are unaffected by the presence of other common mycotoxins. Robustness studies co-varied assay timing (-20%, 60% compared to the standard assay), temperature (18-30 degrees C), and sample volume (+/- 20% compared to the standard assay). Judged against the acceptance criteria, results were unaffected by these changes. The QuickTox Kit for QuickScan Aflatoxin assay is a user-friendly and reliable method for determination of total aflatoxins.
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