Abstract
The method of preparation of infectious ribonucleic acid (RNA) from poliovirus and the factors that influence its degree of infectivity for cells in monolayers have been examined in some detail in an attempt to discover why the efficiency of RNA infection was so low. It was found that isolated RNA was quite stable to both phenol and ether extraction, suggesting that there is little degradation of RNA from the procedures used. The isolation of highly infectious RNA preparations from very pure virus demonstrates that its source can be the intact virus particle. A number of factors were found that influenced the efficiency of RNA in infecting human cells in monolayers. The exposure of host cells to hypertonic salt solutions prior to seeding increased their susceptibility to RNA infection to a significant degree. The salt concentration and pH of the RNA inoculum showed the most striking effect. Under optimum conditions the number of plaques formed was found to be directly proportional to the input of RNA. The maximum plaque-forming efficiency of RNA preparations was 1.0% of the virus from which they were removed.
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