Abstract
The spatial distribution of biomolecules is a critical determinant of cellular health and function. Changes to these distributions underlie many pathologies, making their assessment both ubiquitous and important in the life sciences. Despite major advances in microscopy, colocalization analysis, a simple binary assessment based on signal superposition, and subjective visual interpretation of representative images remain widespread practices. These approaches are limited by their insensitivity to small differences and ignorance of intra- and inter-sample heterogeneity.
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