Abstract

Objective To screen for colorectal cancer associated tumor markers. Methods Laser capture microdissection (LCM) was used to obtain interested cells from 20 colorectal cancer (CRC) and paired normal mucosa tissues. The differential proteins between the microdissected tumor cells and normal mucosa epithelia were analyzed by acetylation stable isotope labeling coupled with fourier-transform ion cyclotron resonance mass spectrometer (FT-ICR-MS). Bioinformatics tools were used for cluster analysis of the differential proteins. Results 137 proteins were differentially expressed by at least 2-fold, including 67 proteins up-regulated and 70 down-regulated in cancer. According to IPA(ingenuity pathway analysis)of the differential proteins, 7 reliable functional networks including cell growth and proliferation, amino acid metabolism, inflammatory response, embryonic development, cell differentiation, molecular transport, and cytoskeletal components were obtained. Conclusions The strategy combining LCM, stable isotope labeling analysis and LTQ-FT MS was effective to profile proteomic changes in CRC cells. Key words: Colorectal neoplasms; Proteomics; Tumor markers, biological; Microdissection

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