Abstract
To investigate the role of TCR signaling in the exit of CD4<sup>+</sup> T cells from cell cycle, we took advantage of a low frequency TEa T cell adoptive transfer technique as well as the Y-Ae mAb to interrupt Ag/MHC recognition before the completion of clonal expansion. Termination of TCR signaling after 36 h of Ag exposure caused an immediate reduction in cell size and deceleration of G<sub>1</sub>—>SG<sub>2</sub>M phase cell cycle progression. As a consequence, clonal expansion in the absence of durable TCR signaling decreased by two-thirds. Thus, CD4<sup>+</sup> T cells scan for the presence Ag throughout their clonal expansion response, and continuously adjust their rate of cell growth and G<sub>1</sub>—>S phase transition to match their intensity of TCR signaling.
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