Abstract
A capillary gas chromatographic method is described for the quantitative analysis of cutin acid methyl esters (CAMEs) obtained by methanolytic depolymerization of plant cutins. The errors resulting from differential losses during diethyl ether extraction of four major CAMEs from the reaction mixture are evaluated using partition coefficients ( K). K values for the system diethyl ether—water strongly depend on the polarity of the CAMEs and the amounts of methanol present. The losses during sample work-up can be corrected for by factors calculated from K values and phase ratios. 2,2-Dimethoxypropane is used instead of solid desiccants for drying diethyl ether saturated with water. Correction factors for the differential response of the gas—liquid chromatographic system to the four CAMEs investigated are determined. It is shown that without correction the actual amounts of CAMEs would be underestimated by 6.2–21.3%.
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