Abstract
Silybin is a characterising compound present in the plant Silybum marianum, used as an hepatoprotective. A simple, accurate and sensitive HPLC method was developed for estimation of silybin. The chromatographic separation was carried out on RP C18 (Waters) column (250 × 4.6 mm, 10 μm) using mobile phase, phosphate buffer: water (65:35 % v/v) pumped at a flow rate of 1 ml/min. The eluent was monitored at 288 nm. The method is specific and linear over the range of 10-200 ng/ml. The method was statistically validated for precision, accuracy, robustness and recovery. The HPLC method can be applied for identification and on of silybin in herbal extracts of Silybum marianum. Key words: HPLC, mother tincture, quantitative estimation, silybin, Silybum marianum
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