Abstract
Background: SERPINA4/kallistatin isa multifunctional protein expressed from the liver; itsconcentration in blood circulation reflects the degree of liver dysfunction and may serve as a diagnostic/prognostic biomarker for chronic liver diseases (CLD). Materials &methods: Antibodies specific for SERPINA4/kallistatin were used for the development ofanenzyme-linked immunosorbent assay (ELISA). For correlative studies, blood samples frompatients withcirrhotic liver and healthy patients were collected from R.L. Jalappa Hospital & Research Centre, Kolar. Results: Interference of other SERPINs was ruled outusing westernblot analysis. Quantitative ELISA was developed using monospecific antibodies as capture antibodies. Conclusion: The accuracy of thedeveloped ELISA was determined by inter- and intra-assay precision. Linearity was definedusing a spiked sample with serial dilutions. Reduced levels of SERPINA4/kallistatin were observed in patients withCLDcompared with healthy controls.
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