Abstract
Chromatographic researches conducted have allowed determination of the flavonoids presence in raw materials of Salvia farinacea Benth. They were rutin, quercetin, hyperoside, and 5 phenol carbonic acids: caffeic, p-coumaric, ferulic, gallic, and cinnamic acids. The quantitative content of the sum of flavonoids in Salvia farinacea Benth. was conducted by the method of differential spectrophotometry, and it amounted to 0.55-0.60% in terms of rutin. The research conducted made it possible to expand the scientific data of phenolic compounds of Salvia farinacea Benth. and to offer the possibility of recycling, monitoring procurement and targeted use of Salvia farinacea Benth. herb in pharmacy and medicine.
Highlights
Pyatigorsk Medical and Pharmaceutical Institute – branch of Volgograd State Medical University of the Ministry of Health of Russia, Pyatigorsk
The quantitative content of the sum of flavonoids in Salvia farinacea Benth. was conducted by the method of differential spectrophotometry, and it amounted to 0.55-0.60% in terms of rutin
The research conducted made it possible to expand the scientific data of phenolic compounds of Salvia farinacea Benth. and to offer the possibility of recycling, monitoring procurement and targeted use of Salvia farinacea Benth. herb in pharmacy and medicine
Summary
Для тонкослойной хроматографии использовали пластинки «Сорбфил ПТСХ-АФ-АУФ» и «Силуфол УФ». The results of the qualitative analysis of the alcohol extract from the grass of Salvia farinacea Benth. Рисунок 1 – Хроматограмма фенольных соединений травы шалфея мучнистого Figure 1 – Chromatogram of phenolic compounds of the grass of Salvia farinacea Benth. Using HPLC method we have discovered 20 substances of phenolic nature in the grass of Salvia farinacea Benth Of this amount 4 phenolic compounds were identified (coffeic, p-coumaric, ferulic, and cinnamic), and three flavonoids (rutin, hyperoside, and quercetine). The flask was connected to the reversed condenser and heated on a boiling bath during 30 minutes, Таблица 3 – Результаты качественного анализа спиртового извлечения из травы шалфея мучнистого методом ВЭЖХ Table 3 – The results of a qualitative analysis of the alcohol extract from the grass of Salvia farinacea Benth. MAU×мин / Peak square, mAU×min spond to the chromatographic maximums, nm Кофейная кислота / Cof-
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