Abstract
To establish a quantitative detection method of culturable Salmonella in water sample,the multiple tube fermentation(MTF) was used to preliminarily select the viable bacteria in which culturable Salmonella are predominant after water concentration by membrane adsorption-elution method.According to the conserved region of invA gene,Salmonella positive strain was identified by colony-PCR with universal primer pairs.The concentration of Salmonella in water sample was calculated by most probably number(MPN) method.This method has good specificity to all Salmonella species,and no cross-reactivity was observed using the genome of other bacteria as PCR template.The validation data of the artificial polluted water and secondary effluent samples indicated that MTF-colony PCR method was accurate,labor-saving and could reflect the activity of Salmonella in water samples.
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