Abstract
Quantitative analysis of thin-layer chromatograms with low 14 C-activities by silver activation and mercury labeling of the autoradiograms The concentration of 14C-labeled sugars (glucose, fructose, and xylose) on thin-layer chromatograms was determined with autoradiograms (contact method; eight days' exposure). Since photodensitometric measurements did not give the precision required, the silver content of the radiograms was determined directly by neutron activation analysis. This method reduces error and facilitates the analytical procedure but requires a long decay time. The precision of the measurements was increased by application of a mercury multiplication solution ( 203Hg-labeled) on the autoradiograms. The mercury activity can be determined immediately after exposure. The graph obtained by plotting the logarithm of the concentration against the mercury activity has a linear region from 10 −4 to 10 −2 μCi.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
