Abstract
A method for the determination of pregnenolone and 17-hydroxypregnenolone in human serum is described which uses high-performance liquid chromatography as a prepurification step followed by radioimmunological quantitation. As to specificity and practicability, the present technique is superior to previously reported methods. Chromatographic assessment of unspecific pregnenolone and 17-hydroxypregnenolone immunoreactivities arising in the ether extracts of normal serum samples clearly emphasizes the necessity of efficient chromatographic isolation of the steroids prior to immunoassay, if specific estimation is to be made. Normal values and physiological changes of serum pregnenolone and 17-hydroxypregnenolone accord well with the data already published in literature.
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Schedule a callMore From: Journal of Chromatography B: Biomedical Sciences and Applications
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