Abstract
The sequence (IdoA2SO 3-GalNAc4SO 3) n contributes to the HCII-mediated inhibition of thrombin by dermatan sulfate (DS). This sequence clearly results from the 13C NMR spectrum and can be quantified by the signal C 1-H of IdoA2SO 3 in the 1H NMR spectrum. A linear correlation has been found between the content in the disulfated disaccharide ΔDi-2,4diS obtained by enzymatic demolition with ABC lyase, the percentage content in IdoA2SO 3 quantified by 1H NMR, and the HCII-mediated activity of dermatan sulfates from beef mucosa and pig skin. DSs have been obtained also from pig mucosa and contain an amount, not negligible, of ΔDi-4, 6diS. This disulfate disaccharide contributes to the activity expressed by the IdoA2SO 3-GalNAc4SO 3 sequence. The analytical techniques HPLC and 1H NMR, applied to the currently performed analyses of DS, are described and discussed.
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