STAR Protocols | VOL. 3
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Quantification of ruffle area and dynamics in live or fixed lung adenocarcinoma cells

Publication Date Jun 1, 2022

Abstract

Ruffles are actin-rich membrane protrusions implicated in actin reorganization and initiation of cell motility. Here, we describe methods for measuring and analyzing ruffle dynamics in live cells and average ruffle area per cell in fixed samples. The specific steps described are for the analysis of A549 lung adenocarcinoma cells, but the protocol can be applied to other cell types. The protocol has applications for dissecting the signaling events linked to ruffling. For complete details on the use and execution of this protocol, please refer to Cooke etal. (2021).

Concepts

Dynamics In Live Cells Lung Adenocarcinoma Cells Actin Reorganization Specific Steps Initiation Of Motility Quantification Of Dynamics Actin-rich Protrusions Live Cells Cell Types Average Area

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No potential conflict of interest was reported by the authors. The conception and design of the study, acquisition of data, analysis and interpretatio...

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