Abstract
pressive effects of MSCs in vitro. Splenocytes from C57BL/6 mice were co-cultured with MSCs from WT C57BL/6 mice (1:20 ratio of MSC to splenocytes) in the presence of anti-CD3, with or without Dex, and proliferation was assayed by H-Tdr incorporation. (B) Total nitrates in the supernatant of the cultures from (A) were assayed by modified Griess reagent. (C-D) Proliferation of human PBMCs co-cultured with human MSCs (1:10 ratio of MSCs to PBMCs) in the presence of anti-CD3 and anti-CD28, with or without dexamethasone or methylprednisolone, respectively. Values are shown as mean SEM. Representative of five independent experiments. S50 Poster Abstracts
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