Abstract
Mangiferin is a natural xanthonoid with various biological activities. Quantification of mangiferin in fruit peel, pulp, and seed kernel was carried out in 11 Chinese mango (Mangifera indica L.) cultivars. The highest mangiferin content was found in the peel of Lvpimang (LPM) fruit (7.49 mg/g DW). Efficient purification of mangiferin from mango fruit peel was then established for the first time by combination of macroporous HPD100 resin chromatography with optimized high-speed counter-current chromatography (HSCCC). Purified mangiferin was identified by both HPLC and LC-MS, and it showed higher DPPH• free-radical scavenging capacities and ferric reducing ability of plasma (FRAP) than by l-ascorbic acid (Vc) or Trolox. In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H2O2-induced stress. Cells treated with mangiferin resulted in significant enhanced cell survival under of H2O2 stress. Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.
Highlights
Mango (Mangifera indica L.), the most important fruit in Anacardiaceae family, is a tropical fruit with high nutritional and medicinal value
The ethyl acetate–n-butanol–water (4:1:5) solvent system showed ideal K value of 1.76 for mangiferin, which resulted in good separation of mangiferin from the resin-refined sample
The structure of the purified compound could be identified as mangiferin (C19H18O11). Both the HPLC and LC-MS data confirmed the purified compound to be mangiferin. These results demonstrated that HPD100 resin chromatography combined with optimized high-speed counter-current chromatography (HSCCC) resulted in efficient purification of mangiferin from LPM peel
Summary
Mango (Mangifera indica L.), the most important fruit in Anacardiaceae family, is a tropical fruit with high nutritional and medicinal value. It originated from the Asia Indo-Burmese region approximately 4000 years ago, and is commercially grown in more than 87 countries [1]. Mangiferin is a heat-stable molecule and a natural pharmacologically active phytochemical that was found to have various bioactivities, such as anti-inflammation [7], anti-diabetic [8], immunomodulatory [9], anti-tumor [10,11], and antioxidant [12,13] It can promote endothelial cell migration during the angiogenesis and may have promising prevention and therapeutic potentials on vascular diseases [14]. The present study was designed to quantify the mangiferin content of 11 Chinese mango cultivars and to isolate mangiferin from mango peel, using a combination of macroporous resin chromatography and HSCCC for evaluation of its antioxidant activity and protective effect on human umbilical vein endothelial cells (HUVEC) under H2O2-induced stress
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