Abstract
The aim of this study was to evaluate the quality of long-term cryopreserved umbilical cord blood (CB) units for hematopoietic cell transplantation (HCT). The recovery of the number of total nucleated cell (TNC), hematopoietic progenitor cells (HPCs; CD34+ cells, colony-forming units-granulocyte/macrophages [CFU-GMs]), and the percentage of viable cells, CD34+ CD38- cells, and CD34+ CXCR4+ cells of CB units cryopreserved for 10years for HCT were examined. Eighteen CB units cryopreserved for 10years (as the study group) and for 1month (as the control group), respectively, were analyzed. The recovery rate of TNC, CD34+ cells and CFU-GMs were 88.72±16.40, 68.39±18.37 and 42.28±38.16% for the study group and 80.17±14.46, 72.67±20.38 and 49.61±36.39% for the control group (p=0.106, p=0.513 and p=0.559, respectively). There were no significant differences in the recovery rate of TNC, CD34+ cells and CFU-GMs between the study group and the control group. The mean basal percentage of viable cells, CD34+ CD38- cells, and CD34+ CXCR4+ cells after thawing were 83.69±9.45, 9.11±4.13 and 81.65±10.82% for the study group. These results indicate that long-term cryopreservation does not negatively affect the quality of CB units for HCT.
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