Abstract
Several investigators have already performed EM studies of tRNA; however, they did not report any statistical assessment of their EM micrographs, and it appears that their preparative procedures may have been limiting in this regard. In contrast, the reproducibly uniform distributions of tRNA particles in the EM micrographs obtained by the procedures described in the accompanying paper allow us to readily identify and characterize tRNA by EM in a quantitative manner. In order to exploit this ability, we have conducted experiments wherein monomers and dimers of tRNA were examined.We have synthesized covalently bonded dimers of tRNA where the monomeric units were linked to each otherviatheir 3′ termini. The 3′ terminal ribose moieties of two monomers were cross-linked with a dihydrazide to produce the dimer. The dimer was purified after synthesis by gel-filtration chromatography (Fig. 1). The preparatory procedure for EM visualization described in the accompanying paper gave sufficient resolution so that the dimer could be readily distinguished from the monomeric starting material (compare Fig. 2a (dimer) with Fig. 2c (monomer)).
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