QTL Analysis of Yield-related Traits using an Advanced Backcross Population Derived from Common Wild Rice (<em>Oryza rufipogon</em> L)

Abstract

Wild rice ( Oryza rufipogon L) is recognized as an important germplasm that has abundant genetic diversity and specific characters. Many of rice breeders attempt to utilize elite wild rice for rice genetic improvement and breeding program to broaden genetic background of cultivating rice. Although There are some reports that wild rice germplasms were successfully applied in rice breeding program, it is definitely depend on what we understand the traits in particular complex traits. In this research the goal was to identify alleles for yield and yield related components. An advanced backcross BC 3 population was generated by a cross between recurrent parent named Yuexiangzhan and donor parent named G52-9, a wild rice collected in Gaozhou of Guangdong . This population was evaluated for 10 agronomic traits related to yield and yield related components. Forty three QTLs of ten traits were detected based on phenotypic data in this study. There are thirty eight QTLs of eight traits detected in continuing two years and thereinto nine QTLs consistently detected in two continuing years. It is also confirmed that nine QTLs mapped on the same or adjacent regions conferring the same traits by previous research. Two QTLs, qGYP-2-1 and qGYP-3-1 mapped on chromosome 2 and 3 can increase the yield of Yuexiangzhan by 40.05% and 49.04%, respectively, contributed from wild rice. Furthermore, the results show that many of detected alleles are likely associated with the trait with wild rice genetic background, which imply that Gaozhou wild rice should be used as excellent gene donor in rice breeding program.