Abstract
Protein derived from purple wheat bran was hydrolyzed sequentially using alcalase proteases for the production of antioxidant peptides. Purple wheat bran protein (PWBP) hydrolysates were fractionated using size-exclusion (G-25) and ion-exchange chromatography methods to identify the structure of antioxidant peptides. The free radical scavenging activity of peptides purified from PWBP hydrolysates was evaluated using superoxide anion radical-scavenging activity and determination assays of Trolox equivalent antioxidant capacity (TEAC). Results demonstrated that purple wheat bran peptide F4-4 exhibited the highest antioxidant activity among other hydrolysates. F4-4 was further identified as Cys-Gly-Phe-Pro-Gly-His-Cys, Gln-Ala-Cys, Arg-Asn-Phe, Ser-Ser-Cys, and Trp-Phe by high performance liquid chromatography (HPLC) spectrometer coupled with Orbitrap Elite™ mass spectrometer (LC–MS/MS). Antioxidant peptides 2 and 4 showed improved stability when the temperature was lower than 80 °C. These peptides also demonstrated good digestive stability in vitro system by simulating gastrointestinal digestion.
Highlights
Antioxidants are substances that counteract the oxidation reaction caused by free radicals
Enzymatic hydrolysis of Purple wheat bran protein (PWBP) by various proteases The degree of hydrolysis (DH) of purple wheat bran protein (PWBP), which was enzymatically hydrolyzed by alcalase, flavourzyme, papain, neutral enzyme, and trypsin, as well as the peptide yield and Trolox equivalent antioxidant capacity (TEAC) of PWBP hydrolysates, were investigated in this study (Table 1)
Results showed that the peptide yield and the TEAC of PWBP hydrolysates prepared from alcalase were remarkably higher than those of the other enzymes’ hydrolysates
Summary
Antioxidants are substances that counteract the oxidation reaction caused by free radicals. Considerable attention has been provided to natural antioxidants due Peptides derived from natural sources are natural antioxidants These antioxidants have been the focus of research interest because of their potential health benefits associated with low molecular weight, low cost, high activity, functional properties, and safety [10, 11]. Bioactive peptides can act as inhibitors of lipid peroxidation to scavenge free radicals directly [11] and prevent damages associated with oxidative stress in humans with regular consumption [9]. Several antioxidant properties of these peptides, Zhao et al BMC Chemistry (2020) 14:58 including their capability to inactivate ROS, scavenge free radicals, and chelate pro-oxidative transition metals [15, 16], have been described
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