Abstract
This chapter focuses on the purification of lectins and determination of their carbohydrate specificity. A number of procedures employed for the purification of the different lectins are basically similar. A common to virtually all lectin purification schemes is affinity chromatography on immobilized sugars, which exploits the ability of lectins to combine with carbohydrates specifically and reversibly. Hemagglutination is routinely assayed with native or modified erythrocytes from humans or other animals, usually rabbits and sometimes sheep. Blood group-specific lectins are tested with a panel of typed human erythrocytes. In plants, lectins often occur as a group of closely related proteins with the same carbohydrate specificity, or isolectins. Different molecular species of the lectin may also result from intramolecular aggregation, possibly occurring during purification.
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