Abstract
Purification of anthocyanins derived from black kidney bean (Phaseolus vulgaris L.) by column chromatography and simulated moving bed (SMB) methods was investigated, and the anthocyanins of black kidney bean were identified. The SMB had advantages over column chromatography in processing efficiency, operation cost, and automation degree in contrast testing. The best SMB conditions resulted in purity and yield of black kidney bean anthocyanins of 24.61 ± 0.21% and 87.85 ± 0.32%, respectively. The half maximal inhibitory concentration (IC50) of 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity and 2,2ʹ-azinobis-(3-ethylbenzthiazoline-6-sulphonate) radical (ABTS+∙) scavenging activity was 0.95 and 2.14 of refined anthocyanins, respectively, indicating strong antioxidant capacity. Three anthocyanins were detected and identified by UPLC-Triple-TOF/MS from black kidney bean skins: delphinidin-3-O-glucoside, petunidin-3-O-glucoside, and malvidin-3-O-glucoside. The experimental results suggested that SMB may help promote industrialization and purification of anthocyanins from colored kidney beans as well as from other plant materials.
Highlights
Kidney bean (Phaseolus vulgaris L.) is a widely cultivated legume crop in China and has important nutritional and medicinal value
LongYun no. 4) were soaked at 30°C for 8 h, and the skin was obtained. e black kidney bean anthocyanins (BKBA) was extracted by the method of Li et al [36]: the bran was extracted by an ultrasonic processor (JinBaiTe, China) twice with 70% (v/v) ethanol and 0.6% hydrochloric acid was added for 20 min
To the best of our knowledge, this is the first report on the purification of BKBA by simulated moving bed (SMB)
Summary
Kidney bean (Phaseolus vulgaris L.) is a widely cultivated legume crop in China and has important nutritional and medicinal value. E utilization of kidney beans will increase with the development of these studies and result in discarding a lot of skin. E skin of colored kidney beans possesses superior antioxidative activity compared with white kidney beans because it contains anthocyanins [12, 13]. Most studies have focused on the antioxidant characteristics [14, 15] and the effect of processing methods on the bioactivity of anthocyanins of kidney beans [16], but research is rare on the purification method of anthocyanins derived from colored kidney beans. Some integration and combination technologies have been used to purify anthocyanins, and the process efficiency has been improved, but there is still some gap before industrialization [29,30,31]. Some integration and combination technologies have been used to purify anthocyanins, and the process efficiency has been improved, but there is still some gap before industrialization [29,30,31]. e continuous chromatography system is a type of simulated moving bed (SMB) chromatography and is an efficient and advanced purification technology.
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