Abstract
Purification and Characterization of d-Lyxose Isomerase
Highlights
The metabolism of n-lyxose by Aerobacter aerogenes PRL-R3 involves isomerization to u-xylulose followed by phosphorylation to n-xylulose 5-phosphate [1, 2]
The enzyme which catalyzes the first reaction in the sequence, n-lyxose isomerase, is of interest because it initiates the degradation of an unnatural pentose in an organism which can utilize as a sole carbon source at least 7 of the 8 aldopentoses [2] and most of the 16 aldohexoses
Kinetic and specificity studies and the inability to demonstrate n-lyxose isomerase in cells grown on substrates other than u-lyxose indicate that this enzyme is elaborated to metabolize the unnatural pentose Dlyxose
Summary
The metabolism of n-lyxose by Aerobacter aerogenes PRL-R3 involves isomerization to u-xylulose followed by phosphorylation to n-xylulose 5-phosphate [1, 2]. The enzyme which catalyzes the first reaction in the sequence, n-lyxose isomerase, is of interest because it initiates the degradation of an unnatural pentose in an organism which can utilize as a sole carbon source at least 7 of the 8 aldopentoses [2] and most (and possibly all) of the 16 aldohexoses.. The present communication, which describes the purification and partial characterization of n-lyxose isomerase from A. aerogenes PRL-R3, indicates that a different situation exists for n-lyxose.
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