Abstract
Pulsed electrochemical detection (PED) coupled to reversed‐phase high performance liquid chromatography is applied to the detection of aryl‐ and alkylglycosides. PED is used to detect these compounds sensitively without the need of derivatization. Detection limits using an optimized waveform are typically 80–40 and 400–200 nM for aryl‐ and alkylglycosides, respectively. For arylglycosides, PED proved generally to be comparable to absorbance detection at 254 nm (300–5 nM) and 204 nm (40–20 nM). The advantages of PED over UV detection are highlighted for alkylglycosides, for which optical detection at 204 nm produced no analytical signal. This work is applied to the determination of a commercial biological surfactant known as Elugent™, which is used to solubilize cell membrane proteins.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Liquid Chromatography & Related Technologies
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
