Abstract
Sixty-eight strains of Gram-negative rod-shaped bacteria which grow well on the surfaces of ordinary culture media but fail to produce acids in Kligler's iron agar were studied. By using the method of Hugh and Leifson these strains were divided into following five groups.Group 1 No action on glucose.Group 2: Produce acid from glucose oxidatively.Group 3: Produce acid from glucose and lactose oxidatively.Group 4: Produce acid from glucose, lactose and sucrose oxidatively.Group 5: ferment glucose anaerobically with acid production.Genus Pseudomonas is included in group 2 and differentiated by motility test and cytochrome oxidase test from Bacterium anitratum, which is included in group 3.The significance of the method of Hugh and Leifson is discussed in comparison with the other identification methods of Gram-negative rod-shaped bacteria.
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