Abstract
Method for production and regeneration of Acetobacter pasterianus protoplasts is described. The protoplasts were obtained by treatment of cells with lysozyme in protoplast buffer at pH 8.0 with different osmotic stabilizers. The protoplasts were regenerated on Acetobacter medium with various osmotic stabilizers. Maximum protoplast formation was obtained in protoplast buffer with NaCl (0.5M) as an osmotic stabilizer and lysozyme (0.5mg/ml). Addition of mutanolysin did not show enhancement in protoplast formation. Maximum protoplast regeneration was obtained on Acetobacter medium with NaCl (0.5M) as an osmotic stabilizer. This is, to our knowledge, the first report on protoplast formation and efficient regeneration in case of Acetobacter pasteurianus.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: American Journal of Bioengineering and Biotechnology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
