Abstract
Results form /sup 1/H NMR studies of /sup 15/N-labeled Escherichia coli tRNA/sub f//sup Met/ indicated an unambiguous assignment for the G-U pair and detection of a uridine resonance at 11.4 ppm. Results indicated that signals for only 24-25 imino protons are clearly seen for E. coli tRNA/sub f//sup Met/ at 35/sup 0/C. Since 21 imino hydrogens participate in presumably more stable secondary interactions, only 3 or perhaps 4 peaks for tertiary hydrogen bonds are visible. Our results with /sup 15/N-labeled material indicate that uridine imino hydrogens in s/sup 4/U8-A14, rT54-A58, and G18-psi55-P58 (or U60) account for three of these tertiary signals.
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