Abstract
The rate of K +/H + exchange through bilayer lipid membranes (BLM) induced by nigericin was measured by the method of pH gradient offset according to Antonenko, Yu.N. and Yaguzhinsky L.S. [(1990) Biochim. Biophys. Acta 1026, 236–240]. It was shown that under the conditions of high potassium ion concentration the rate of nigericin-mediated K +/H + exchange increased with an increase in the concentrations of such buffer compounds as citric acid and MES. The concentration dependence was different for citrate and MES. The buffer concentration effect was absent at low potassium ion concentrations. Citrate increased the rate of K +/H + exchange being added to the side of BLM where the K + concentration was higher and had no effect at the opposite side. At High KCl and citrate concentrations, the rate of K +/H + exchange was about 6 times lower in D 2O when compared to H 2O solutions. It is concluded that under certain experimental conditions the overall rate of the K +/H + exchange induced by nigericin is determined by the rate of proton dissociation from nigericin at the membrane—water interface.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
