Proteomics reveals dynamic phagosomal environment during Mycobacterium -HIV co-infection

Abstract

Event Abstract Back to Event Proteomics reveals dynamic phagosomal environment during Mycobacterium -HIV co-infection Rakesh Ganji1, Snigdha Dhali2, Arshad Rizvi1, Srikanth Rapole2 and Sharmistha Banerjee1* 1 University of Hyderabad, India 2 National Centre for Cell Science, India Mycobacterial infection is a major cause for increased mortality in HIV infected patients. In order to understand the coalition between Mycobacteria and HIV that makes these diseases more destructive when confronted together, we undertook a proteomics approach to identify differentially regulated proteins during intra-phagosomal phase of infection in macrophages under mycobacteria-HIV co-infection. A comparative protein expression pattern of host proteins during mycobacterial and mycobacteria-HIV co-infection of macrophages revealed 95 phagosomal and 70 mitochondrial proteins differentially regulated. An extreme cytoskeletal rearrangement was observed with categorical up-regulation of energy metabolism proteins during co-infection. Purinergic receptors, which are capable of adjusting cellular metabolic needs and also help HIV entry, were observed to be upregulated during co-infection. Interestingly, co-infected host cells mounted augmented inflammatory response, as evident from increased manganese superoxide dismutase and intracellular ROS levels. 94 mycobacterial proteins were differentially regulated upon co-infection, majority of which belonged to intermediary metabolism, respiration, cell wall synthesis and information pathways. During co-infection, Mycobacteria showed increased expression of proteins of ESX secretion systems that are instrumental in secretion of virulent proteins. An increased expression of cAMP dependent regulator (Rv2565), Rv1265 (regulated by cAMP dependent regulator) and RecF (DNA repair protein) upon co-infection may protect mycobacteria from increased host ROS levels. Altered expression of toxin-antitoxin family of proteins during mono- and co-infection suggested regulated mycobacterial stress response that may influence programmed cell death. Conclusively, proteomics data suggested a dynamic host-pathogen environment during co-infection leading to identification of critical factors that explained the coalition between HIV and mycobacteria. Keywords: Mycobacterium, HIV, Co-infection, phagosome, Proteomics Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Ganji R, Dhali S, Rizvi A, Rapole S and Banerjee S (2013). Proteomics reveals dynamic phagosomal environment during Mycobacterium -HIV co-infection. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00822 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 24 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Sharmistha Banerjee, University of Hyderabad, Hyderabad, India, sbsl@uohyd.ernet.in Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Rakesh Ganji Snigdha Dhali Arshad Rizvi Srikanth Rapole Sharmistha Banerjee Google Rakesh Ganji Snigdha Dhali Arshad Rizvi Srikanth Rapole Sharmistha Banerjee Google Scholar Rakesh Ganji Snigdha Dhali Arshad Rizvi Srikanth Rapole Sharmistha Banerjee PubMed Rakesh Ganji Snigdha Dhali Arshad Rizvi Srikanth Rapole Sharmistha Banerjee Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.