Abstract
Sclerotinia ginseng is a necrotrophic soil pathogen that mainly infects the root and basal stem of ginseng, causing serious commercial losses. Sclerotia, which are important in the fungal life cycle, are hard, asexual, resting structures that can survive in soil for several years. Generally, sclerotium development is accompanied by the exudation of droplets. Here, the yellowish droplets of S. ginseng were first examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the proteome was identified by a combination of different analytical platforms. A total of 59 proteins were identified and classified into six categories: carbohydrate metabolism (39%), oxidation-reduction process (12%), transport and catabolism (5%), amino acid metabolism (3%), other functions (18%), and unknown protein (23%), which exhibited considerable differences in protein composition compared with droplets of S. sclerotium. In the carbohydrate metabolism group, several proteins were associated with sclerotium development, particularly fungal cell wall formation. The pathogenicity and virulence of the identified proteins are also discussed in this report. The findings of this study may improve our understanding of the function of exudate droplets as well as the life cycle and pathogenesis of S. ginseng.
Highlights
As described in the experimental procedures, by day 6, aerial hyphae were first bestrewed on the potato dextrose agar (PDA) culture in a Petri dish (90 mm) at 20 ◦C in the dark (Fig. 1A)
At the same time the aerial hyphae ceased vegetative growth and started to differentiate, which was consistent with previous reports (Erental, Dickman & Yarden, 2008; Patsoukis & Georgiou, 2007) (Figs. 1B and 2A)
After 8 days of inoculation, the obvious appearance of sclerotium development was observed, with the hyphae coalesced into snowball-shaped mycelia (Figs. 1C and 2B)
Summary
How to cite this article Wang et al (2017), Proteomics research and related functional classification of liquid sclerotial exudates of Sclerotinia ginseng . There has been little research on the function of exudate droplets, studies should be performed due to their physiological significance. Studies have shown that pathogenicity is another important property of exudate droplets, and some proteins may be involved in pathogen development and virulence (Liang, Strelkov & Kav, 2010).
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