Proteomics evidence for kefir dairy in Early Bronze Age China

Abstract

Cheese making has been inferred at several sites in northern Europe as early as the 6th millennium BC and was common in Egypt and Mesopotamia in 3rd millennium BC. However, the remains of ancient cheeses have never been found and recipes of ancient dairy, its production scale, social and economic impact remain poorly understood. Here we present direct proteomics evidence for the production of an earliest known cheese that was found as an organic mass associated with the mummies of Early Bronze Age cemetery of Xiaohe (1980–1450 BC) in Xinjiang, China. Kefir fermentation of ruminant milk by a symbiotic culture of Lactobacillus kefiranofaciens and other lactic acid bacteria and yeasts was the basis of robust, scalable, probiotic, lactose-free dairy and a key technological advance that introduced economic benefits of extensive herding into a semi-pastoral household of the Eastern Eurasia population already in the Early Bronze Age.