Abstract
Drosophila brain has emerged as a powerful model system for the investigation of genes being related to neurological pathologies. To map the proteomic landscape of fly brain, in a high-resolution scale, we herein employed a nano liquid chromatography-tandem mass spectrometry technology, and high-content catalogues of 7,663 unique peptides and 2,335 single proteins were generated. Protein-data processing, through UniProt, DAVID, KEGG and PANTHER bioinformatics subroutines, led to fly brain-protein classification, according to sub-cellular topology, molecular function, implication in signaling and contribution to neuronal diseases. Given the importance of Ubiquitin Proteasome System (UPS) in neuropathologies and by using the almost completely reassembled UPS, we genetically targeted genes encoding components of the ubiquitination-dependent protein-degradation machinery. This analysis showed that driving RNAi toward proteasome components and regulators, using the GAL4-elav.L driver, resulted in changes to longevity and climbing-activity patterns during aging. Our proteomic map is expected to advance the existing knowledge regarding brain biology in animal species of major translational-research value and economical interest.
Highlights
Drosophila brain has emerged as a powerful model system for the investigation of genes being related to neurological pathologies
The reverse process of ubiquitin (Ub) removal from Ub-bound proteins is accomplished by a superfamily of more than 100 deubiquitinating enzymes (DUBs)27,31. 26S proteasome is composed of a 20S proteolytic complex, being capped by one or two 19S regulatory complexes28,32. 20S-CP comprises 14 different αand β-type subunits, stacked in 4 heptameric rings being arranged in an α1–7, β1–7, β1–7 and α1–7 architecture
Through employment of a nano Liquid Chromatography - tandem Mass Spectrometry proteomics approach, we have identified a total of 2,335 individual proteins in the single-transgenic elav.L-GAL4/+ female adult (2–3-day-old) Drosophila brain
Summary
Drosophila brain has emerged as a powerful model system for the investigation of genes being related to neurological pathologies. We have recently shown, through engagement of the elav.L-GAL4/UAS and RNAi genetic platforms, that neuronal cell-specific targeting of CCS (copper chaperone for SOD1) gene affected climbing activity of Drosophila female flies during aging but not their longevity[39], suggesting that -at least- in this system the activation of RNAi machinery alone cannot affect aging.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
