Abstract
Dense protein complexes such as those found in the immunological synapses of antigen-activated lymphocytes are sites of critical biological activity, but their study is complicated by the large number of protein species present. Array tomography is a proteomic imaging method capable of addressing this high-dimensional problem through iterative immunostaining, but would be difficult to apply to spatially-restricted regions of interest such as complexes within the immunological synapse that form in the area of contact between lymphocytes and antigen-presenting surfaces. We have developed a novel variant of this technique which embeds a thin (∼500 nm) slice of material in LR White, making proteomic multiplexing possible. We are using this method with physically unroofed resting and activated B cells to study signaling in antigen-receptor microclusters in B cell plasma membranes. Here we present this method, and our current work in its application to the B cell membrane.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
