Abstract
Many studies have been now focused on the promising approach of fungal endophytes to protect the plant from nutrient deficiency and environmental stresses along with better development and productivity. Quantitative and qualitative protein characteristics are regulated at genomic, transcriptomic, and posttranscriptional levels. Here, we used integrated in-depth proteome analyses to characterize the relationship between endophyte Piriformospora indica and Brassica napus plant highlighting its potential involvement in symbiosis and overall growth and development of the plant. An LC-MS/MS based label-free quantitative technique was used to evaluate the differential proteomics under P. indica treatment vs. control plants. In this study, 8,123 proteins were assessed, of which 46 showed significant abundance (34 downregulated and 12 upregulated) under high confidence conditions (p-value ≤ 0.05, fold change ≥2, confidence level 95%). Mapping of identified differentially expressed proteins with bioinformatics tools such as GO and KEGG pathway analysis showed significant enrichment of gene sets involves in metabolic processes, symbiotic signaling, stress/defense responses, energy production, nutrient acquisition, biosynthesis of essential metabolites. These proteins are responsible for root’s architectural modification, cell remodeling, and cellular homeostasis during the symbiotic growth phase of plant’s life. We tried to enhance our knowledge that how the biological pathways modulate during symbiosis?
Highlights
The vegetable oil production has been crossed approximately 87 million metric tons every year
On 28th-day post inoculation, branched roots, more lateral rootlets with numerous root hairs were well developed in P. indica treated B. napus (Fig. 2A,B)
By using label-free quantification with following the parameters such as 95% confidence level (p-value ≤ 0.05), fold change ≥2, at least one unique peptide as a cut offs with significant method ANOVA, we identified fungal and plant proteins which are differentially expressed after efficient colonization of P. indica in rapeseed plant’s root
Summary
The vegetable oil production has been crossed approximately 87 million metric tons every year. Endophytes help in improving the plant health, growth, development and productivity. These endophytes receive essential organic carbon forms from the host for survival. Fungus supports enhanced water and nutrient uptake, protect from various biotic, abiotic stresses and plant pathogens for better growth of the host plant[5,6,7,8]. P. indica confers enhanced vegetative growth, stress tolerance, resistance to plant pathogens in wide host range from monocot to dicot plant species without host specificity. Our group has studied the interaction between P. indica and B. napus, resulted in significant enhancement of agronomic parameters, biomass and nutrient elements contents. Leaf proteomics of P. indica colonized barley plant under salt and water stress stated that P. indica induces a systemic response by altering physiological and metabolic capacity in colonized plants improving tolerance to stresses[16,17]
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