Abstract
Fish skin is a vital organ that serves a multitude of functions including mechanical protection, homeostasis, osmoregulation and protection against diseases. The expression of skin proteins changes under different physiological conditions. However, little is known about differences in protein expression among various body sites in naïve fish. The objectives of this work is to study potential differences in protein and gene expression among dorsal, caudal and ventral regions of lumpfish skin employing 2D gel based proteomics and real-time PCR and to assess structural differences between these regions by using Alcian blue and Periodic acid Schiff stained skin sections. The proteins collagen alfa-1, collagen alfa-2, heat shock cognate 71 kDa, histone H4, parvalbumin, natterin-2, 40S ribosomal protein S12, topoisomerase A and topoisomerase B were differentially expressed among the three regions. mRNA expression of apoa1, hspa8 and hist1h2b showed significant differences between regions. Skin photomicrographs showed differences in epidermal thickness and goblet cell counts. The ventral region showed relatively high protein expression, goblet cell count and epidermal thickness compared to dorsal and caudal regions. Overall, this study provides an important benchmark for comparative analysis of skin proteins and structure between different parts of the lumpfish body.
Highlights
Skin is the largest and outermost organ in the fish body
The present study aims to investigate the differences in histological skin sections using Alcian blue and Periodic acid Schiff staining, protein expression using two dimensional gel electrophoresis coupled with tandem mass spectrometry, and the gene expression of selected genes using qPCR in lumpfish skin among different body regions
In the present study we used 2D gel based proteomics and LC-MS/MS to identify skin proteins of lumpfish, seventeen of these were differentially expressed among the dorsal, caudal and ventral regions of lumpfish skin
Summary
Skin is the largest and outermost organ in the fish body. It is the first protective barrier between its internal organs and the external aquatic environment[1]. The present study aims to investigate the differences in histological skin sections using Alcian blue and Periodic acid Schiff staining, protein expression using two dimensional gel electrophoresis coupled with tandem mass spectrometry, and the gene expression of selected genes using qPCR in lumpfish skin among different body regions. To our knowledge this is the first study approaching a differential protein expression among various body sites in fish
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
