Abstract
Proteomic approaches were applied in four grain developmental stages of the Chinese bread wheat Yunong 201 and its ethyl methanesulfonate (EMS) mutant line Yunong 3114. 2-DE and tandem MALDI-TOF/TOF-MS analyzed proteome characteristics during middle and late grain development of the Chinese bread wheat Yunong 201 and its EMS mutant line Yunong 3114 with larger grain sizes. We identified 130 differentially accumulated protein spots representing 88 unique proteins, and four main expression patterns displayed a dynamic description of middle and late grain formation. Those identified protein species participated in eight biochemical processes: stress/defense, carbohydrate metabolism, protein synthesis/assembly/degradation, storage proteins, energy production and transportation, photosynthesis, transcription/translation, signal transduction. Comparative proteomic characterization demonstrated 12 protein spots that co-accumulated in the two wheat cultivars with different expression patterns, and six cultivar-specific protein spots including serpin, small heat shock protein, β-amylase, α-amylase inhibitor, dimeric α-amylase inhibitor precursor, and cold regulated protein. These cultivar-specific protein spots possibly resulted in differential yield-related traits of the two wheat cultivars. Our results provide valuable information for dissection of molecular and genetics basis of yield-related traits in bread wheat and the proteomic characterization in this study could also provide insights in the biology of middle and late grain development.
Highlights
Hexaploid wheat (Triticum aestivum, 2n = 6× = 42, AABBDD) is one of the most important cereals that provides a large proportion of essential nutrients in the human diet
Identification, and Classification of Differentially Accumulated Proteins during Grain Development Yunong 201 and Yunong 3114 had similar proteomic profiles at the four stages according to the two-dimensional gel electrophoresis (2-DE) protein maps that were extracted from both samples (Figures 2, 3)
There were more than 1000 gel spots detected over the gel, and 173 spots were detected that displayed altered abundance, which were analyzed by mass spectrometry
Summary
Two-dimensional electrophoresis (2-DE) and mass spectrometry (MS) proteomic approaches have been broadly applied to investigate the dynamic expression profiles of proteins during grain development in different plant species, including Arabidopsis (Ruuska et al, 2002; Li et al, 2007), soybean (Li et al, 2012), maize (Méchin et al, 2007), and rice (Thelen, 2009; Zhang et al, 2012). Identification of proteins in the first 2 weeks of grain development stages showed that a total of 10 clusters of genes were examined in bread wheat (Nadaud et al, 2010). The proteomes of hard and soft near-isogenic wheat lines at four grain developmental stages revealed that kernel hardness is related to the amplification of a stress response during endosperm development (Lesage et al, 2012). Proteome characterization of four grain developmental phases in wheat cultivars Jimai 20 and Zhoumai 16 indicated that differences in seed storage proteins were related to different flour quality performance from these wheat cultivars (Guo et al, 2012)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
