Abstract

General Strategies, Shawn Doonan. Preparation of Extracts from Animal Tissues, Shawn Doonan. Protein Extraction from Plant Tissues, Peter R. Shewry and Roger J. Fido. Extraction of Recombinant Protein from Bacteria, D. Margaret Worrall. Protein Extraction from Fungi, Paul Bridge. Subcellular Fractionation of Animal Tissues, Norma M. Ryan. Subcellular Fractionation of Plant Tissues: Isolation of Chloroplasts and Mitochondria from Leaves, Alyson K. Tobin. The Extraction of Enzymes from Plant Tissues Rich in Phenolic Compounds, William S. Pierpoint. Avoidance of Proteolysis in Extracts, Robert J. Beynon and Simon Oliver. Concentration of Extracts, Shawn Doonan. Making and Changing Buffers, Shawn Doonan. Purification and Concentration by Ultrafiltration, Paul Schratter. Bulk Purification by Fractional Precipitation, Shawn Doonan. Ion-Exchange Chromatography, David Sheehan and Richard FitzGerald. Hydrophobic Interaction Chromatography, Paul O'Farrell. Affinity Chromatography, Paul Cutler. Dye-Ligand Affinity Chromatography, D. Margaret Worrall. Lectin Affinity Chromatography, Iris West and Owen Goldring. Immunoaffinity Chromatography, George W. Jack and David J. Beer. Immobilized Metal Ion Affinity Chromatography, Tai-Tung Yip and T. William Hutchens. Chromatography on Hydroxyapatite, Shawn Doonan. Affinity Precipitation Methods, Jane A. Irwin and Keith F. Tipton. Isoelectric Focusing, Reiner Westermeier. Chromatofocusing, Timothy J. Mantle and Patricia Noone. Size-Exclusion Chromatography, Paul Cutler. Fast Protein Liquid Chromatography (FPLC) Methods, David Sheehan. Reversed-Phase Chromatography of Proteins, Bill Neville. Extraction of Membrane Proteins, Kay Ohlendieck. Removal of Detergent from Protein Fractions, Kay Ohlendieck. Purification of Membrane Proteins, Kay Ohlendieck. Lyophilization of Proteins, Ciaran A Fagain. Storage of Pure Proteins, Ciaran A Fagain. Electroelution of Proteins from Polyacrylamide Gels, Michael J. Dunn. Electroblotting of Proteins from Polyacrylamide Gels, Michael J. Dunn. High-Performance Electrophoresis Chromatography, Serge Desnoyers, Sylvie Bourassa, and Guy G. Poirier. Practical Column Chromatography, Shawn Doonan. Index.

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