Abstract
In most hospitals worldwide formalin-fixation and paraffin-embedding (FFPE) is the standard tissue fixation and storage method. The analysis and extraction of full-length and immunoreactive proteins from FFPE tissues was long believed to be impossible due to protein cross-linking during fixation. Nevertheless, in recent years, several research groups have successfully established protocols to extract proteins from FFPE tissues. For use in clinical in vitro diagnostics the extraction protocol should not be too complex and has to be compatible with downstream molecular analysis such as Western-blot, reverse phase protein array, or mass-spectrometry. Here we describe a protocol for protein extraction from FFPE tissues that fulfills these requirements.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
