Abstract
SPECIFIC AIMSHumanUCP3, predominantly expressed in skeletal muscle, plays an important role in the dissipation of energy in human energy metabolism and is therefore linked to obesity and type 2 diabetes. It has been deduced from rodent studies that the extent and direction in which, for example, fatty acid levels, GLUT4 content and physical training affect UCP3 mRNA expression is muscle fiber-type specific. To explore the role of UCP3 in human energy metabolism further, we wanted to examine the abundance of protein expression of UCP3 relative to muscle fiber typology in a direct manner and at the protein level in m. vastus lateralis muscle of healthy controls and type 2 diabetics.PRINCIPAL FINDINGS1. The antibody used here specifically recognizes UCP3 in cryo-sections of human m. vastus lateralis sections as well as in whole muscle homogenatesThe UCP3 antibody was raised against a 20 amino acid (147–166 of human UCP3). Affinity purified serum was tested for specificity in immunofluorescence of H9C2 cells ...
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