Protein differential expression in the latex from Hevea brasiliensis between self-rooting juvenile clones and donor clones

Abstract

To better understand molecular mechanism underlying the difference between self-rooting juvenile clones and donor clones, a proteomic approach was used to profile protein changes in the latex between self-rooting juvenile clones and donor clones. Total soluble proteins were extracted from latex in self-rooting juvenile clones and donor clones. Two-dimensional gel electrophoresis (2-DE) was used to identify proteins that were differentially expressed in self-rooting juvenile clones and donor clones and image analysis was used to determine which proteins were up- or down-regulated. Twenty-four differentially expressed protein spots were identified by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Among the 24 proteins identified, 13 proteins were up-regulated, 11 proteins were decreased in self-rooting juvenile clones. These proteins were classified as carbohydrate and energy metabolism, secondary metabolism, signal translocation, transcriptional regulation-related, protein synthesis and degradation, transport, nucleoside acid process, lipid metabolism. Perhaps, the present study contributes towards an understanding of the molecular mechanism underlying the difference between self-rooting juvenile clones and donor clones.