Abstract
This paper aimed to verify the protective effects of Thuja orientalic extract against oxidative stress caused by H2O2 Rotenone and Paraquat in the keratinocyte(HaCaT cell) of human-derived normal skin for the development of safer health food or functional cosmetic materials. It has been reported that Thuja orientalic has antioxidant effects. In accordance with the cytotoxicity of Thuja orientalic extract against HaCaT cells using MTT, the appropriate concentration was less than 50㎍/mL. Thuja orientalic extract exhibited protective effects against H2O2, rotenone, and paraquat by 86.6%, 86.45%, and 60.24%, respectively, at the concentration of 50㎍/mL. Thuja orientalic extract has protective effects against all three artificial stresses, and more protective effects against H2O2 and rotenone. The analysis results indicated that Thuja orientalic extract had antioxidant effects that could offer protection to the cells from a variety of oxidative stresses. Accordingly, Thuja orientalic extract has a highly applicable value as a functional health food or cosmetic ingredient in the current times in which wellbeing is a priority.
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