Abstract
The present study conducted to test the effect of Nigella Sativa (NS, 5 mg kg -1 of body weight), or γirradiated Nigella Sativa (GRNS) on the changes of blood component profiles, liver, kidney functions and immune cytokines secretion in male Wistar rats. Moreover, the possible protection by propolis (200 mg kg -1 B. W.) on the changes induced by NS and GRNS was examined. Results revealed that both NS and GRNS administration for two weeks induced changes in blo od, GPT, GOT and urea levels and co-administration with propolis significantly ameliorated such change s. Also, liver histology showed numerous vacuolar degeneration and fatty changes in γ-irradiated groups which disappeared in presence of propolis. Kidney histology of NS administered rats showed less lymphocytic infiltration, while GRNS groups showed desquamation in the cytoplasm of the renal tubules, hemorrhage in the renal corpuscle and lymphocytic infiltration which disappeared when propolis given together with GRNS. Finally propolis induced protec tive effect on the changes induced in TNF-α and IL-10 secretion by either NS or GRNS in Wistar rats. In conclusion, the findings of present study clarified the protective effect of propolis on changes induc ed by γirradiated NS on blood, liver, kidney and cytokines changes in Wistar rats.
Highlights
During last decade the medical applications of radiation technology have been increased rapidly due to development of new diagnostics and therapeutic methods using gamma irradiation in medical therapy (Hendee, 1992)
To test the changes induced by administration of either Nigella Sativa (NS) or gamma irradiated NS extract (GRNS) alone or in combination with propolis for extra two weeks
Unlike Hb and red blood cell results, NS treatment showed significantly increasing in white blood cell counts, while propolis (p
Summary
During last decade the medical applications of radiation technology have been increased rapidly due to development of new diagnostics and therapeutic methods using gamma irradiation in medical therapy (Hendee, 1992). Development of protective agents presented new solutions for recovery of undesired tissue damage induced by ionizing radiation (Borek, 2004). Irradiation causes activation of caspase-3 by DNA fragmentation (Epperly et al, 2002). These pathways are common in apoptosis induction by TNF-α and mediation of other cytokines as IL-1 and IL-8 (Epperly et al, 2003a). Its water extract contain thymoquinone and oily substances called monoterpenes (El-Tahir et al, 1993) Both Houghton et al (1995) and Worthen et al (1998), reported anti-cancer and oxidant activity for NS
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