Abstract
Objective The objective of this research is to investigate the protective effects of hypoxia pretreatment on oxygen-glucose deprivation human brain microvascular endothelial cell (HBMVEC) via detecting mitochondrial function and hypoxia-inducible factor-1α(HIF-1α) and succinate dehydrogenase complex iron sulfur subunit B(SDHB) expression in oxygen-glucose deprivation HBMVEC. Methods Cultured HBMVEC were randomly divided into 5 groups: sham group (group A), oxygen-glucose deprivation group (group B), oxygen-glucose deprivation+hypoxia pretreatment group (group C), oxygen-glucose deprivation+hypoxia pretreatment+dimethyloxalylglycine (DMOG)(group D) and oxygen-glucose deprivation+hypoxia pretreatment+2-ME (group E). Then, cell apoptosis was detected by Annexin V kit. ATP content was analyzed by ATP assay kit. The level of HIF-1α and SDHB expression were determined by Western blot. Results Compared with group A, the expression of HIF-1α was up-regulated, ATP content was significantly decreased and cell apoptosis was increased in group B, group C, group D and group E (P<0.05), the expression of SDHB was significantly down-regulated in group B, group C and group E (P<0.05). Compared with group B, the expression of HIF-1α and SDHB was up-regulated, ATP content was significantly increased and cell apoptosis was decreased in group C and group D(P<0.05). Conclusions Hypoxia pretreatment can protect mitochondrial respiration function in oxygen-glucose deprivation HBMVEC via promoting HIF-1α and SDHB expression. Key words: Oxygen-glucose deprivation; Human brain microvascular endothelial cell; Hypoxia pretreatment; Hypoxia-inducible factor-1α; Succinate dehydrogenase complex iron sulfur subunit B
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