Abstract
Disruption of the function of sirtuin1 (SIRT1) has been reported to link with diabetes and metabolic disorders. The aim of this study was to investigate the effect of a modified high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) or continuous endurance training (CET) program on Sirt1 and PGC-1α mRNA gene expression in hepatocytes of high-fat/high-fructose diet-induced diabetes in rats. Twenty-eight male Wistar rats (220 ± 20 g) were randomly divided into four groups of non-diabetic (NDC), diabetic control (received high fat high-fructose diet (HFHFD-DC)), CET (HFHFD-CET), and HIIT (HFHFD-HIIT). Diabetes was induced by feeding rats a high-fat (30%) high-fructose (20%) diet for 25 weeks. Following confirmed hyperglycemia, both HFHFD-CET (60–65% Vo2max) and HFHFD-HIIT (85–90% Vo2max) rats underwent treadmill exercise for 30 min, 5 days/week at 10 m/min for 8 weeks. Thereafter, blood and liver samples were obtained for biochemical studies. For histological analysis, the liver was fixed in 10% formalin, embedded in paraffin, and sectioned (5 μm thickness) for H&E staining. SIRT-1 and PGC-1α mRNA expression were measured by RT-PCR from the liver sample (frizzed sample). All data were presented as mean ± standard error mean and compared the differences by one-way analysis of variances. The alpha level was set at 0.05 (p < 0.05) for statistical difference. The results show that HIIT was more effective than CET to reduce vesicular steatosis. HIIT significantly enhanced the expression of SIRT-1 compared to the HFHFD-DC group (fold change: 1.47, p < 0.047). However, HIIT decreased PGC-1α expression compared to the HFHFD-DC group, but it was not significant (fold change: 1.32 and p < 0.247). HIIT was more effective than CET in SIRT-1 expression augmentation in high-fat, high-fructose diabetic rats which suggests a possible treatment strategy in the context of type 2 diabetes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
